SIRCHIE LV509 Aqueous Leuco Crystal Violet Kit 500

( Brand: Sirchie ), ( Part Type: Kit ), ( Manufacturer Part Number: LV509 ), ( Country/region Of Manufacture: United States )

Review SIRCHIE Aqueous Leuco Crystal Violet Kit 500 Ml #lv509

The Sirchie LV509 Aqueous Leuco Crystal Violet Kit, with a capacity of 500 ml, is a high-performance, laboratory-grade solution designed for the microbiological detection and enumeration of bacteria in various samples, including water, food, and environmental matrices.

This kit employs the Leuco Crystal Violet (LCV) method, which is a widely accepted and standardized technique for the determination of total heterotrophic plate count (THPC) and the most probable number (MPN) method for the estimation of coliforms and E. coli. The LV509 Aqueous Leuco Crystal Violet Kit fulfills the requirements of numerous recognizable standards, such as APHA, AOAC, and ISO.

1. A stable stock solution of Leuco Crystal Violet dye: This component provides a consistent and reliable source of the Leuco Crystal Violet dye, ensuring accurate results. The dye is formulated to remain in its leuco or colorless form until exposed to reducing agents like those produced by microbial metabolism.

2. An activator solution: The activator solution is a necessary component of the LCV method since it comprises reducing agents that trigger the transformation of the Leuco Crystal Violet dye into its final crystalline, purple form, allowing for the visualization and quantification of microbial growth.

3. A set of Petri dishes: The included Petri dishes are specifically designed for this application, ensuring optimal results during the incubation and enumeration of microbial growth.

One essential advantage of the Sircie LV509 Aqueous Leuco Crystal Violet Kit is its ease of use. The technique does not require any complex procedures or specialized equipment. It has a relatively short incubation time, typically between 2 and 4 hours, making it an excellent option for routine microbiological analysis in various industries, including food, water, pharmaceutical, and environmental monitoring applications.

The kit's components are packaged safely for shipping and stability, ensuring they arrive in optimal condition. Each component is clearly labeled and clearly defined expiration dates to maintain the highest level of accuracy and consistent results throughout the lifetime of the kit. With rigorous quality control measures and compliance with recognized industry standards, this Sircie LV509 Aqueous Leuco Crystal Violet Kit is a reliable and efficient solution for microbiological analysis.

Pros and Cons of Buying Sirchie LV509 Aqueous Leuco Crystal Violet Kit (500 ml)

The Sirchie LV509 Aqueous Leuco Crystal Violet Kit is a crime laboratory reagent commonly used for the detection and enumeration of gram-negative bacteria in various forensic and microbiological investigations. This section presents the advantages and disadvantages of purchasing this product.

1. Highly Selective: This kit selectively stains gram-negative bacteria, making it an essential tool in the identification of potential contaminants in a wide array of samples, including water, food, and environmental samples.

2. Easy to Use: The kit is straightforward to use, requiring minimal hands-on time for preparation and staining procedures. Essentially, only three steps preparation, incubation, and decolorization are required for accurate results.

3. Large Sample Volume: The 500 ml kit allows for the processing of a significant number of samples, making it a cost-effective solution for laboratories handling a high volume of samples.

4. Long Shelf-life: The Sirchie LV509 Aqueous Leuco Crystal Violet Kit has a long shelf-life, reducing the need for frequent replacements and providing laboratories with continuous access to this essential reagent.

1. Limited Staining Scope: This kit only stains gram-negative bacteria, making it less versatile than other kits that can detect both gram-positive and gram-negative bacteria.

2. Possible False Positives: In some instances, leuco crystal violet can produced false positives when staining gram-positive bacteria, especially if they are spore-forming or have significant amounts of polysaccharides. This may require additional confirmatory testing to distinguish between false and true positives.

3. Staining Artifacts: Occasional staining artifacts or inconsistent results might be observed due to temperature or other environmental factors, possibly requiring technicians to repeat staining procedures.

The Sircie LV509 Aqueous Leuco Crystal Violet Kit offers several advantages, including its selectivity for gram-negative bacteria, ease of use, large sample volume, and long shelf-life. However, the limitations of this kit, such as its inability to stain gram-positive bacteria and the possibility of false positives, must be considered before purchasing. Ultimately, the decision to buy the Sirchie LV509 Aqueous Leuco Crystal Violet Kit should depend on the specific requirements and goals of the laboratory.

If a laboratory predominantly analyzes gram-negative bacteria or needs a cost-effective solution for processing a high volume of samples, the Sircie LV509 Aqueous Leuco Crystal Violet Kit is an excellent choice. However, if the laboratory requires a more versatile kit that can stain both gram-positive and gram-negative bacteria or has a low volume of samples, other alternative options should be explored.

part # lv509 price

• $46.50

specifications carechomp:

• brand: Sirchie
• part type: Kit
• manufacturer part number: LV509
• country/region of manufacture: United States

general carechomp:

• condition: New with box
• Health Beauty > Health Care > Other Health Care Supplies

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• rating: 99.6%
• # reviews: 595444

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• availability: In Stock
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• started: February 7, 2025